Decreasing the trauma and increasing the efficiency of brain inoculate A-beta seeding by ultrasound mediated delivery of seeds to the brain.
Within the field of protein aggregation diseases a lot of different animal models exists that develop plaque (aggregates) in the brain of time, sometimes more than a year. Often, brain homogenates are injected intra cordially to "seed" the development of aggregates and speed up the development of the disease. This procedure is time consuming and rather invasive. Ultrasound mediated blood-brain barrier opening is a well established technology and does not cause any detectable short- or long-term adverse effects. In this study we propose that intra venous injections of the homogenate will be sufficient for seeding if the blood-brain barrier is opened by ultrasound.
60 Tg-Swe mice will be used. It is not expected that they will perceive anything but mild discomfort during the experiment.
This is a pilot and the number of animals are accordingly low. The goal of the study is to develop a new method to deliver brain homogenates to the brain. If successful, this new method will be a major refinement to currently used methods.
60 Tg-Swe mice will be used. It is not expected that they will perceive anything but mild discomfort during the experiment.
This is a pilot and the number of animals are accordingly low. The goal of the study is to develop a new method to deliver brain homogenates to the brain. If successful, this new method will be a major refinement to currently used methods.