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In efforts to find more sustainable and effective lice control methods in salmon farming, techniques that expose fish to fresh- and brackish water salinities that are harmful to salmon lice are being tested. This animal ethics application is for a laboratory experiment that will determine the duration and frequency of exposures at different brackish water salinities that are effective at reducing lice. The knowledge will guide the development of salinity-based technologies against salmon lice in salmon farming.

We expect the animals in this experiment to experience minimal distress during a typical laboratory salmon lice infection challenge, and brackish water salinity treatments that this fish species as post-smolts are known to withstand (an anadromous fish).

Social benefits of developing more sustainable and effective methods for salmon lice control include improved fish production destined for human consumption, and less impacts on wild salmon populations which support an important recreational fishing industry and a small commercial fishery.

A total of 1440 post-smolt salmon for this experiment.

We have investigated avenues for replacement, reduction and improvement with regard to this experiment. In short, the study animal cannot be replaced as they are needed to produce the salmon lice stages to be studied (attached copepodid and chalimus stages). We have minimised the number of animals in the small tank experiment to a level that statistically robust results can still be achieved. We have improved the experiment, building methods from previous studies, so that best practices in lice and salmon research are used (e.g. removal of one handling of fish step, that has been used in previous studies).