Functional analysis of STAMPs in breast cancer
Our laboratory is studying genes that are implicated in the progression of breast cancer; recently we have found that six transmembrane proteins of prostate (STAMPs) that are involved in this regard. STAMP genes expression is associated with multiple clinic-pathological parameters. Our current in vitro studies confirmed that dysregulation of STAMP genes (STAMP1 and STEAP) expression confers development and progression of breast cancer. By subcutaneously engrafting breast cancer cells into female immune-deficient mice, we will determine whether the effect of STAMPs on breast cancer cell growth in vivo is similar to what we have observed in vitro. We expect that the data generated from this experiment, together with the other data we have, will uncover the role of STAMP1 in breast cancer pathobiology and will lead to development of novel biomarkers for breast cancer.
Regarding the 3R, in vitro experiments have already been performed. To investigate whether the results obtained in vitro are applicable to an in vivo setting, experiments in live animals are necessary. Eight animals per group (totally 72 mice ) should give sufficient data to obtain statistical significance of the results based on our/others experience in similar experiments. Smaller groups of animals may not give enough statistical power. The cell lines used will be tested for contaminating microorganisms and infectious agents such as mycoplasma and hepatitis. The animals will be anesthetized using isofluran prior to cell injection to prevent discomfort to the animals.
Regarding the 3R, in vitro experiments have already been performed. To investigate whether the results obtained in vitro are applicable to an in vivo setting, experiments in live animals are necessary. Eight animals per group (totally 72 mice ) should give sufficient data to obtain statistical significance of the results based on our/others experience in similar experiments. Smaller groups of animals may not give enough statistical power. The cell lines used will be tested for contaminating microorganisms and infectious agents such as mycoplasma and hepatitis. The animals will be anesthetized using isofluran prior to cell injection to prevent discomfort to the animals.