Role of AQP4 in health and disease: Non channel functions of AQP4
Our laboratory has its focus on the role of aquaporin water channels. Aquaporin-4 (AQP4) is the predominant brain water channel. We have generated a transgenic mouse strain lacking AQP4 (AQP4-KO), which we have used in several of our projects.
In this project we will be looking at a newly generated mouse with a single mutation in the AQP4 gene. The mouse is generated by Transgensenteret at IMB, and the mutation is based on a patient with a similar mutation. As we don't know to what extent the mice will have a phenotype, we will be investigating all the organs where AQP4 is expressed, including the central nervous system ( brain, spinal cord), eyeballs, kidneys, heart and skeletal muscle. The mutant mice will be backcrossed with WT C57/Bl6 mice for 7 generations.
To begin with, we will perform investigate the morphology (microscopy, immunohistochemistry), mRNA and protein expression (Western blotting and qPCR), and generation of primary cell cultures.
We will need to build up a breeding colony of Het x Het over time. We will also perform behavioral tests and functional experiments of brain and heart. Details of these experiments will follow in future applications. This application is just for building up a breeding colony of a mouse with a new mutation and performing ex vivo experiments (harvesting organs)-
In this project we will be looking at a newly generated mouse with a single mutation in the AQP4 gene. The mouse is generated by Transgensenteret at IMB, and the mutation is based on a patient with a similar mutation. As we don't know to what extent the mice will have a phenotype, we will be investigating all the organs where AQP4 is expressed, including the central nervous system ( brain, spinal cord), eyeballs, kidneys, heart and skeletal muscle. The mutant mice will be backcrossed with WT C57/Bl6 mice for 7 generations.
To begin with, we will perform investigate the morphology (microscopy, immunohistochemistry), mRNA and protein expression (Western blotting and qPCR), and generation of primary cell cultures.
We will need to build up a breeding colony of Het x Het over time. We will also perform behavioral tests and functional experiments of brain and heart. Details of these experiments will follow in future applications. This application is just for building up a breeding colony of a mouse with a new mutation and performing ex vivo experiments (harvesting organs)-